ABSTRACT
Aim: To determine the effect of cell therapy on the intensity of lipid peroxidation processes in the liver, kidneys and lungs of rats of different ages under conditions of experimental cranio-skeletal trauma (CST).
Materials and Methods: In the experiments, 129 white male Wistar line rats of different age groups were used: immature rats aged 100-120 days and weighing 90-110 g; mature rats aged 6-8 months and weighing 180-200 g; and old rats aged 19-23 months and weighing 300-320 g. In each age group, CST was modeled under thiopental sodium anesthesia. The control rats were only injected with thiopental sodium anesthesia. For the purpose of correction, cryopreserved neuroblast cells (NBC) from Wistar line rats were injected intravenously at a dose of 0,5 ml*106 cells in groups of injured rats of different age groups. Additionally, in separate groups, rat mesenchymal stem cells (MSC) were injected intraperitoneally at a dose of 0,25 ml*105 cells per rat. The animals were taken out of the experiments using anesthesia after 14 days by total heart bleeding. The content of thiobarbituric acid reagents was determined in 10 % extract of liver, kidney and lung homogenate.
Results: In 14 days after the CST was applied in rats of different age groups, a significantly higher content of TBA-active lipid peroxidation products was observed compared to control groups of rats of the corresponding age. Under conditions of NBC monotherapy in experimental groups of different aged rats, a decrease in the content of TBA-active lipid peroxidation products occurred in the liver, kidney and lungs, but the result was statistically significant only in the group of mature rats. The injection of a combination of NBC and MSC for the purpose of correction was accompanied by a significantly greater effect compared to rats without correction. After 14 days of post-traumatic period, the content of TBA-active lipid peroxidation products in the liver and kidneys of different aged rats significantly decreased. In these organs, as well as in the lungs of mature rats, the combination therapy showed a greater antioxidant effect compared to rats without correction and rats with NBC monotherapy. The obtained results shed light on the specificity of the systemic antioxidant effect of combined NBC and MSC cell therapy in rats of different age groups with CST, which should be taken into account in the development of cell transplantation strategies under conditions of severe combined trauma. 
Conclusions: Combined transplantation of NBC and MSC during acute period of CST among rats of different age groups is accompanied by a systemic antioxidant effect, which is manifested by a significant decrease in the content of TBA-active lipid peroxidation products in the liver, kidney and lungs, the degree of which is mostly marked among mature rats.
KEY WORDS: cranio-cerebral trauma, skeletal trauma, liver, kidney, lungs, oxidative stress, cell therapy

ABSTRACT
Aim: The aim of the study was to determine the impact of cranioskeletal trauma (CST) on the development of endogenous intoxication syndrome in rats of different ages.
Materials and Methods: The experiments involved 147 white male Wistar rats of different age groups. The first experimental group included sexual immature rats aged 100-120 days. The second group includes sexually mature rats aged 6-8 months. The third group included old rats aged 19-23 months. In all experimental groups, CST was modelled under thiopentalonatrium anaesthesia. The control rats were only injected with thiopentalonatrium anaesthesia. The animals were withdrawn from the experiments under anaesthesia after 1, 3, 7, 14, 21 and 28 days by total bleeding from the heart. In blood serum, the content of fractions of molecules of middle mass was determined at a wavelength of 254 and 280 nm (MMM254, MMM280).
Results: As a result application of CST in rats of different age groups, an increase in the serum content of MMM254 and MMM280 was observed with a maximum after 14 days and a subsequent decrease by 28 days. At all times of the experiment, the indicators were statistically significantly higher compared to the control groups. The degree of growth of the MMM254 fraction after 1, 7 and 14 days was statistically significantly higher in sexual immature rats, and after 21 and 28 days – in old rats. In old rats after 21 and 28 days of the post-traumatic period, the content and degree of growth of the MMM280 fraction in the blood serum were also significantly higher.
Conclusions: Modelling of CST in rats of different age groups is accompanied by the development of endogenous intoxication syndrome, which is manifested by the accumulation of MMM254 and MMM280 fractions in the blood serum with a maximum after 14 days of the experiment. The content of the serum fraction of MMM254 in sexual immature rats in the dynamics of experimental CST exceeds other age groups after 1, 7 and 14 days, in old rats the content of the studied MMM fractions is significantly higher after 21-28 days.
KEY WORDS: traumatic brain injury, skeletal injury, endogenous intoxication of the kidney, age, molecular of middle mass 
 

Introduction. Previous researches and the bitter experience of other countries affected by armed conflicts show that at least one in five people will have negative consequences for mental health, and one in ten will experience these consequences at the level of moderate severity or severe illness. In recent decades, much attention has been paid to mental health problems around the world, primarily due to their high prevalence and medico-social consequences, which leads to an increase in demand for psychotropic drugs. Therefore, it is advisable to conduct a marketing research of psychotropic drugs, the results of which will make it possible to establish trends in the development of the market of the investigated group of drugs and assess the mental health of young people in wartime.

Background: Surgical intervention is associated with psychological and physical stress for the patient. The severity of surgical stress is also influenced by anesthetics, analgesics, and the choice of anesthesia method. Heart rate variability (HRV) is a universal reaction of the entire body to any impact.

Methods: 92 patients aged 67.47±14.39 years (23% women) who underwent surgical treatment of hernias of the anterior abdominal wall took part in the study. Patients were divided into 3 groups according to the method of anesthesia: 1st group (n= 32) operated under general multicomponent anesthesia, 2nd group (n= 30) with neuraxial (spinal or epidural) anesthesia, 3rd group (n= 30)–regional blockades (the rectus abdominis sheath and transverse abdominal plane). A multidisciplinary decision was made on the specifics of planned surgical intervention and anesthetic support. The 3rd group included the majority of …

UDC 616.12-008.46:616.127-005.8:616-005]-07-08

Cardiogenic shock (CS) is a clinical syndrome resul琀椀ng from a sharp decrease in cardiac output, which leads to hypoperfusion of vital organs and 琀椀ssues. Despite the achievements of pharmacological therapy, mortality in CS remains high. In this regard, the role of temporary mechanical circulatory support (TMCS) methods is increasing, which allows for stabilising hemodynamics, ensuring adequate organ perfusion, and reducing the load on the myocardium. Such devices can serve as a bridge to restoring heart func琀椀on, provide long-term mechanical support, or facilitate transplanta琀椀on. Over the past decade, signi昀椀cant progress has been noted in the development of TMCS technologies, among which intraaor琀椀c balloon counterpulsa琀椀on (IABP), microaxial pump systems (Impella), transseptal ventricular assist devices (TandemHeart) and extracorporeal membrane oxygena琀椀on (ECMO) are the most widely used. The purpose of this study is to examine various op琀椀ons for modern methods of temporary mechanical circulatory
support, to compare their availability, advantages, and disadvantages, as documented in modern literature, to op琀椀mise the intensive care of pa琀椀ents with acute heart failure. Biblioseman琀椀c, compara琀椀ve and systema琀椀c analysis methods were used. The results of this study are based on
data from the analysis of modern literature, as well as the results of randomised trials and meta-analyses devoted to the study of modern methods of temporary mechanical circulatory support. The use of MCS devices in pa琀椀ents with progressive heart failure unresponsive to conven琀椀onal intensive care management provides hemodynamic support and stabiliza琀椀on and increases pa琀椀ent survival. Rather than viewing these devices as compe琀椀ng technologies, it is essen琀椀al to understand each device individually to leverage its unique proper琀椀es in various clinical situa琀椀ons. Early use of MCS devices in CS is associated with be琀琀er outcomes because they mi琀椀gate the adverse e昀昀ects of systemic hypoperfusion on target organs and reduce the need for inotropes/vasopressors, thereby minimising myocardial oxygen consump琀椀on and improving microcircula琀椀on.
Key words: cardiogenic shock, temporary mechanical circulatory support, intra-aor琀椀c balloon counterpulsa琀椀on, Impella, TandemHeart, extracorporeal membrane oxygena琀椀on