UDC 547.655.6:612.112.94:615.35:612.015.11

It is known that 1,4-naphthoquinone derivatives form the basis of a wide range of pharmaceuticals with diverse biological activities. A newly synthesized compound of this group – 11-(furan-2-yl)-9-hydroxy-3,11-dihydro-2H-benzo[6,7]thiochromeno[2,3-d]thiazole-2,5,10-trione, designated by Les-6400 laboratory code, is noteworthy for its drug-like properties. The aim of the study was to determine the parameters of glutathione antioxidant system in isolated human peripheral blood lymphocytes treated with Les-6400. Saponin-permeabilized lymphocytes from the blood samples of healthy male volunteers aged 20–44 years were used in the study. A significant dose-dependent increase in the lipid peroxidation process was observed upon lymphocytes exposure to Les-6400 at concentrations of 10^–5–10^–3 M. At the concentrations studied no effect of Les-6400 on GSH level was observed, while in the 10^–4–10^–3 M concentration range the activity of glutathione antioxidant enzymes was markedly affected: the activity of both glutathione peroxidase and glutathione reductase was increased, while that of glutathione-S-transferase was reduced. Thus, Les-6400 had a pronounced effect on the pro/antioxidant status of blood lymphocytes.

УДК: 612.015.11:616.69-008.1:616-001

Introduction. To study the predictive ability of using the quantitative value of the level of a single indicator to detect oxidative stress, ROC analysis was performed with the calculation of the optimal cut-off threshold selected according to the criterion of maximum balance between sensitivity and specificity.The aim of this research is to analyze the diagnostic sensitivity and specificity of pro- and antioxidant system indicators in men injured as a result of combat operations.Materials and methods. 116 samples of peripheral blood lymphocytes and serum from men, which were divided into two groups by age, were analysed for the sensitivity and specificity of pro- and antioxidant system indicators.Results. The results of ROC analysis for indicators of the pro- and antioxidant system of lymphocytes and peripheral blood serum demonstrated their predictive ability for detecting oxidative stress. Malondialdehyde of blood lymphocytes for the age group 20–39 years, reduced serum glutathione and total glutathione of blood lymphocytes for all examined men were characterized by excellent discriminatory ability. Malondialdehyde of blood lymphocytes for the age group 20–39 years showed itself as a highly sensitive marker with a slightly lower specificity value. Reduced glutathione of blood serum of men of all age groups is characterized as a highly specific marker with a slightly lower sensitivity value. Total glutathione of blood lymphocytes is simultaneously characterized by high sensitivity and specificity for all examined men.Conclusions. The concentration of malondialdehyde in blood lymphocytes and the levels of total and reduced glutathione in blood serum were the most significant predictors for the detection of oxidative stress based on the ROC analysis. These markers demonstrate high sensitivity and specificity as indicators of the pro- and antioxidant balance.Keywords: erectile dysfunction, combat trauma, pro- and antioxidant system, ROC analysis.