Keywords: tumor necrosis factor alpha; vaccination with autoleukocytes; chronic hepatitis B.

Methylobacterium mesophilicum - is a gram-negative bacillus, most often isolated in medical establishments. For humans M. mesophilicum is considered to be conditionally pathogenic flora. Cases of infection with this agent in humans occur, as a rule, in immunodeficient individuals. A clinical description of a rare case of infection with Methylobacterium mesophilicum with development of acute meningitis in 26-year-old patient is presented in the article. A considerable decrease in bactericidal activity of neutrophils was detected in the patient. So far cases of meningitis due to Methylobacterium mesophilicum have not been described.

The article describes the case of a septic form of a listeriosis with multiple abscesses of the liver. Given clinical example illustrates the severe course of listeriosis with the development of septicemia and lesions of internal organs. Despite the adequate etiotropic and pathogenetic treatment for listeriosis meningoencephalitis, there was a lethal outcome of the disease. The polymorphism of clinical manifestations and the range of course variants often make it difficult to diagnose listeriosis, which in turn requires a more thorough examination of patients as well as carrying out all relevant bacteriological and serological tests.

ABSTRACT
The aim: Was a trial of intradermal immunization with native autoleukocytes as a curative vaccine.
Materials and methods: Thus, 3-shot series vaccination by means of intradermal injection of autoleukocytes with 30 – 40 days interval was conducted for patients who, in spite of continuous (at least 2 years) therapy with nucleotide analogue, experienced HBV DNA reproduction. For this procedure, 80 – 100 ml of a patient’s heparinized venous blood was being precipitated at the temperature 37о С for 120 – 140 minutes, after blood plasma was being centrifuged at 450g for 8 minutes. The precipitate was resuspended in 1 – 1.5 ml of blood serum and injected intradermally in the region of the back.
Results: Viral load decreased in all patients even after single immunization; it was possible to achieve a negative result by ultrasensitive PCR method in 23.33% of patients in the group of patients who did not respond adequately to antiviral therapy.
Conclusions: In patients with chronic hepatitis B, intradermal immunization with autoleukocytes has a significant impact on intensity of virus replication. It is confirmed by a considerable reduction of DNA HBV amount in patients, in whom antiviral therapy was stopped before immunization
 KEY WORDS: chronic hepatitis B, therapy with nucleotide analogue, autoleukocyte vaccination

The aim: Analyzing literature sources, to assess possibilities of using the method of intradermal immunization with native autoleukocytes to treat different diseases, to investigate the areas of usage, efficacy and expediency of the technique in clinical practice.
Materials and methods: Analysis of literature sources associated with intradermal immunization with native autoleukocytes.
Conclusions: The possibilities of using the method of intradermal immunization with native autoleukocytes in the treatment of various diseases are considered in the literature review. Intradermal immunization with autoleukocytes is one of the methods of personalized medicine. The application of the method results in normalization of the immune system condition as well as suppression of autoimmune and inflammatory processes. It also reduces the synthesis of
pro-inflammatory cytokines and strengthens cellular antiviral immunity in a number of viral infections. It is proved, in particular, that the method reduces the synthesis of cryoglobulins, the formation of antithyroid antibodies, normalizes the level of tumor necrosis factor alpha, as well as reduces extrahepatic manifestations of chronic hepatitis and increases the effectiveness of antiviral therapy in patients with viral hepatitis B. Considering that immunization with
native autoleukocytes has no contraindications, it can be used in many diseases.
KEY WORDS: antithyroid antibodies, antiviral therapy, cryoglobulinemia, antinuclear antibodies, immunization with autoleukocytes, tumour necrosis factor α