УДК: 616–093+547.789

The present microreview provides access to recent advances in the synthetic approaches to novel thiazolo[4,5-b]-pyridines developed over the last years. This second part presents the overview and analysis of modern synthetic techniques for thiazolo[4,5-b]pyridine bicyclic scaffold construction starting from pyridine derivatives followed by thiazole heterocycle annulation

УДК: 616–093+547.789

As a result of the furfural reaction with diazonium, salts 1a-h the arylfuran-2-carbaldehydes 3a-h were synthesized. In the reaction of Wilgerodt-Kindler, arylfuran-2-carbaldehydes with sulfur and aryl piperazines was prepared 1-aryl-4-[(5-aryl-2-furyl)carbonothioyl]piperazines. The structures of target compounds 5a-l were confirmed by using 1H NMR spectroscopy and elemental analysis. The antimicrobial activity of the synthesized substances was evaluated by the value of the MIC and minimum fungicidal and bactericidal concentration. The findings exhibited that the compounds
possessed moderate antimicrobial potential. In vitro anticancer activity assessment on the full panel

UDC 547.789:615.276:576.851:616.34-002]-074-092.4/.9

Nonsteroidal anti-inflammatory drugs, which are widely used in the treatment of diseases accompanied by pain and fever, can cause diseases of the gastrointestinal tract and are associated with disturbances of the intestinal microbiota. The search for new compounds that could affect the community of microorganisms, exhibiting antimicrobial and anti-inflammatory effects, is an important task of modern medicine and veterinary medicine. One of the promising molecules that have such effects are 4-thiazolidinone derivatives. The aim of this study was to analyze the effect of the newly synthesized compound Les6490 and drug nimesulide on the intestinal wall microbiota of rats in vivo under the conditions of Freund’s adjuvant-induced inflammatory process. The study of the effect of the above-mentioned drugs on the intestinal microbiota in vivo was carried out on a biomodel of rats, which were intragastrically administered with the test substances for two weeks. The study material was the parietal mucos of the small intestine, the microbiome of which was studied using 16S rRNA sequencing. Metagenomic analysis made it possible to analyze the types of microorganisms in experimental groups with induced inflammation (groups A and AL) and without it (groups K, L, N). It was established that the composition of the microbiome of the intestinal tract of rats changes under the conditions of induced inflammation and under the action of the compound Les6490 (groups A and L) in comparison with the control group (group K). The influence of Les6490 on the intestinal tract microbiome composition in rats is similar to that of nimesulide, but the effect is more …

Nonsteroidal anti-inflammatory drugs (NSAIDs), used in the treatment of numerous diseases, can cause a number of side effects, the main of which is an ulcerogenic effect on the mucous membranes of the gastrointestinal tract. NSAIDs can also cause side effects in the hepato-biliary, cardiovascular, excretory systems, affect the functioning of the liver and lungs [1]. The mechanism of action of NSAIDs is based on the inhibition of the activity of cyclooxygenase (COX), which exists in two main isoforms COX-1 and COX-2, differ structurally and functionally [2, 3]. COX-1 is a constitutive isoform that is expressed throughout the body and synthesizes prostaglandins involved in numerous physiological processes. In particular, with the participation of COX-1, prostaglandins are formed. They regulate the secretion of hydrochloric acid and stimulate the formation of protective mucus in the stomach, influence the process of proliferation and intercellular integration in various organs, regulate vascular homeostasis, platelet aggregation, and kidney function. COX-2 is an inducible form of the enzyme, which expression increases during inflammation, ensuring the synthesis of relatively large concentrations of prostaglandins, that act as the inflammation mediators [4, 5]. Considering the numerous side effects of currently existing NSAIDs, the development of new anti-inflammatory drugs devoid of side effects is actively underway. In this sense, the compounds capable of simultaneous inhibiting the cyclooxygenase and lipoxygenase (LOX) pathways of arachidonic acid metabolism are of significant interest. Dual-acting COX/LOX inhibitors could provide multiple...

UDC 616.314/.716.1-001-085:616.15 

 Restoring the connection between the tooth and the periodontium is the primary goal of complex treatment, which involves the formation of new bone and cement, restoring the periodontal ligament and blood supply to the pulp from the surrounding tissues. The study aimed to investigate the effect of platelet-rich plasma as a medium for temporarily storing a dislocated tooth on the regeneration and reconstruction of its tissues after replantation of an extracted tooth compared to saline and water. In this study, 30 adult male Wistar rats were used, and a tooth was extracted and then replanted. A fibroblast cell activity analysis was performed after 72 hours to determine cell viability. Cells incubated in platelet-rich plasma medium had a higher percentage of cell viability (82.7±5.1), followed by saline (74.3±4.2) with statistical significance (p>0.001). The minimum percentage was recorded in cells in the medium with tap water (68.8±3.9). The pulp, dentin and cementum of the group where the teeth were placed in platelet-rich plasma showed normal histological features, demonstrating optimal tissue remodelling and attachment to the surrounding bone. There were no signs of root resorption. In summary, this study used several temporary storage procedures and evaluated changes in histological events that occurred during the storage of extracted teeth and replantation until the time of recording of the success of the replantation. The study's results indicate the use of platelet-rich plasma as a storage medium, as it significantly preserves the viability of dental cells compared to water and saline solutions.

Key words: teeth dislocations, tooth replantation, permanent teeth, platelet-rich plasma, traumatic injuries of the maxillofacial area.