Background. It is known about wide variety of individual reactions to stress explained by genetics factors. On the other hand, it is also known about aerobic fitness variability between individuals. From the above it follows the hypothesis that inter-individual differences in normal conditions determine the characteristics of the body's response to acute stress. The purpose of this study is to test the hypothesis. Material and methods. The experiment is at 58 rats (28 males) Wistar line. Animals were tested for resistance to hypoxic hypoxia and aerobic muscular performance by swimming test. On the basis of the received data two qualitatively equivalent groups in a ratio 10/48 were formed. After a week of recovery over the next 10 days, one animal remained intact and 5 other rats were exposed to water-immersion and restraint stress. The next day after stress, the ECG recorded and some endocrine, metabolic and immune parameters determined as well as erosive-ulcerative lesions of the gastric mucosa evaluated. Results. Four clusters by hypoxic and swimming tests were created retrospectively: normal resistance to hypoxia and muscular endurance (n=11); moderately reduced resistance to hypoxia and normal swimming test (n=25); drastic increased swimming test and normal hypoxic test (n=3); significantly increased resistance to hypoxia and normal swimming test (n=9). Each cluster is characterized by specific (correctness of classification 100%) post-stress changes in 6 neuro-endocrine, 12 immune, 10 metabolic and 2 ECGs parameters, as well as the index of damage to the gastric mucosa. The swimming test determines the post-stress state of the registered parameters by 63,8%, the hypoxic test - by 57,5%, and taken together - by 79,1%. Conclusion. The post-stress neuro-endocrine, immune and metabolic parameters as well as injuries of myocardium and gastric mucosa in rats are determined significantly by innate muscular endurance and resistance to hypoxia.
The first scientific publication on general adaption syndrome, or as we know today biologic stress has been published in Nature in 1936 by the 29-year old Hans Selye.1 Szabo et al2 in the anniversary review "Stress" is 80 Years Old” conclude that despite the extensive and multidisciplinary research on stress during the last 80 years, a lot of basic and clinical research is needed to better understand the manifestations, central and peripheral molecular regulators of stress response, especially the modes of prevention/management of distress or its transformation into eustress and the treatment of stress-related diseases.
In the vast majority of publications on stress, the HPA-, HPG- and autonomous systems are the objects of research, while the place in the general adaptation syndrome of such important hormones as calcitonin and PTH has been studied only in a few publications. Another methodological shortcoming of most studies is that the subjects of analysis are limited to a single neuro-endocrine system.
Therefore, we set ourselves the goal: to analyze relationships between some adaptation hormones, HRV, calcitonin, and PTH as well as electrocardiogram and gastric mucosal damage in naїve and post stressed rats.
Background. Earlier, we found a close canonical correlation between parameters of gas discharge visualization (GDV) and principal neuroendocrine factors of adaptation.
The purpose of this study is to elucidate the relationship between GDV and immunity parameters.
Material and research methods. We observed twice 10 women and 10 men aged 33-76 years without clinical diagnose. In the morning in basal conditions at first registered kirlianogram by the method of GDV by the device “GDV Chamber” (“Biotechprogress”, SPb, RF). For further analysis the following parameters were selected: Area, Shape Coefficient as ratio Square Length of outward contour gas discharge image to its Area as well as Entropy of contour in Right, Frontal and Left projections registered both with and without polyethylene filter. Estimated also Energy and Asymmetry of virtual Chakras. Then registered routine parameters of cellular and humoral Immunity. Results processed by method of canonical analysis, using the software package “Statistica 5.5”.
Results. According to the value of the canonical correlation coefficient R with GDV parameters, the immunity parameters are arranged in the following order: IgA (0,716; p=0,005), CD8+CD3+ Tc-lymphocytes (0,646; p=0,004), IgG (0,645; p=0,002), IgM (0,622; p=0,0001), “active” T-lymphocytes (0,572; p=0,007), CD4+CD3+ Th-lymphocytes (0,566; p=0,003), CIC (0,491; p=0,018), 0- lymphocytes (0,457; p=0,036), CD16+ NK-lymphocytes (0,396; p=0,043), CD22+ B- lymphocytes (0,439; p=0,105). The integral canonical correlation between the parameters of GDV and Immunity was very strong (R=0,994; p<10-4).
Conclusion. Between parameters of Immunity and parameters of GDV exist strong canonical correlation suggesting relevance and informativenes this method.
Key words: Gas Discharge Visualization, Cellular and Humoral Immunity, Relationships.