ABSTRACT
Aim: To determine the effect of cell therapy on the intensity of lipid peroxidation processes in the liver, kidneys and lungs of rats of different ages under conditions of experimental cranio-skeletal trauma (CST).
Materials and Methods: In the experiments, 129 white male Wistar line rats of different age groups were used: immature rats aged 100-120 days and weighing 90-110 g; mature rats aged 6-8 months and weighing 180-200 g; and old rats aged 19-23 months and weighing 300-320 g. In each age group, CST was modeled under thiopental sodium anesthesia. The control rats were only injected with thiopental sodium anesthesia. For the purpose of correction, cryopreserved neuroblast cells (NBC) from Wistar line rats were injected intravenously at a dose of 0,5 ml*106 cells in groups of injured rats of different age groups. Additionally, in separate groups, rat mesenchymal stem cells (MSC) were injected intraperitoneally at a dose of 0,25 ml*105 cells per rat. The animals were taken out of the experiments using anesthesia after 14 days by total heart bleeding. The content of thiobarbituric acid reagents was determined in 10 % extract of liver, kidney and lung homogenate.
Results: In 14 days after the CST was applied in rats of different age groups, a significantly higher content of TBA-active lipid peroxidation products was observed compared to control groups of rats of the corresponding age. Under conditions of NBC monotherapy in experimental groups of different aged rats, a decrease in the content of TBA-active lipid peroxidation products occurred in the liver, kidney and lungs, but the result was statistically significant only in the group of mature rats. The injection of a combination of NBC and MSC for the purpose of correction was accompanied by a significantly greater effect compared to rats without correction. After 14 days of post-traumatic period, the content of TBA-active lipid peroxidation products in the liver and kidneys of different aged rats significantly decreased. In these organs, as well as in the lungs of mature rats, the combination therapy showed a greater antioxidant effect compared to rats without correction and rats with NBC monotherapy. The obtained results shed light on the specificity of the systemic antioxidant effect of combined NBC and MSC cell therapy in rats of different age groups with CST, which should be taken into account in the development of cell transplantation strategies under conditions of severe combined trauma. 
Conclusions: Combined transplantation of NBC and MSC during acute period of CST among rats of different age groups is accompanied by a systemic antioxidant effect, which is manifested by a significant decrease in the content of TBA-active lipid peroxidation products in the liver, kidney and lungs, the degree of which is mostly marked among mature rats.
KEY WORDS: cranio-cerebral trauma, skeletal trauma, liver, kidney, lungs, oxidative stress, cell therapy