Aim: To investigate the effect of succinic acid on the humoral component of the immune system in rats.
Materials and Methods: The study was conducted on two groups of mature non-linear white rats (males) of similar weight (200-270 g, aged 6-8 months),with 5 animals in each group. The control group was fed a standard diet with free access to water throughout the experiment. Rats in the experimental group were subcutaneously injected with a 0,1% solution of succinic acid in a liposomal emulsion at a dose of 20 cm³ for five days. The state of the humoral component of the immune system was assessed by measuring serum immunoglobulins A, M, and G using solid-phase enzyme-linked immunosorbent assay. Circulating immune complexes were determined in a 40% solution of polyethylene glycol.
Results: Under the influence of succinic acid in liposomal emulsion the content of class A immunoglobulins increased by 44,0% (p<0.01) compared to rats in the control group. The activation of class A immunoglobulin synthesis points to the provision of local immunity of the mucous membranes in the rat’s body. The content of class M immunoglobulins increased by 61,0% (p<0.001) compared to the control group rats. This high activity of class M immunoglobulins indicates their rapid activation in the body of rats in response to primary contact with succinic acid in liposomal emulsion. The content of class G immunoglobulins increased by 36,0% (p<0.05) compared to the control group rats. No clinical deviations from physiological norms were observed in the rats after the use of succinic acid in liposomal emulsion. After the use of succinic acid in liposomal emulsion in the experimental group of rats, the concentration of CICs increased by 15,0% (p<0.05) compared to the control group rats. In our case, the increase in CIC levels is not correlated with clinical manifestations but is a consequence of increased levels of class M and G immunoglobulins.
Conclusions: The succinic acid in liposomal emulsion activates the production of class A, M, G immunoglobulins, circulating immune complexes, it prevents
the development of secondary immunodeficiency and has a positive impact on the humoral branch of the immune system in rats.

UDC: 616. 24-06: 616.248+616.33-002.44)-092: 612.015.11.-08

Bronchial asthma (BA) and stomach ulcer are among the most common human diseases which remain important medical and social problems in the XXI century. There are combinations of pathologies that burden each other in the practical work of the doctor.
The aim of this study was to establish the peculiarities of changes in the processes of lipoperoxidation and antioxidant protection in the lungs with the development of experimental bronchial asthma (EBA) and experimental stomach ulcer (ESU).
Material and research methods. Experimental studies were performed on 55 guinea pigs (males) weighing 180 — 220 g, divided into 5 groups of 9 animals each, except the first (10 animals). Group I (control) included intact guinea pigs, II — animals with experimental asthma and ESU (5th day), III — guinea pigs on the 19th day of the combined model process, IV — animals with EBA and ESU (26th day), V — guineapigs on the 33rd day of EBA and ESU. For the purpose of detailed analysis and interpretation of indicators of prooxidant and antioxidant systems (AOS) in different days of experiment two periods of development of EBA and ESU were conditionally distinguished: early (5th and 19th days of experiment) and late (26th and 33rd days).
The experimental asthma model was reproduced in guinea pigs by the method of VI Babich, gastric ulcer was simulated according to the method of VI Komarov. Condition of free radical oxidation in the lungs was determined by the content of diene conjugates by the method of VG Gavrylov, MI Myshkorudna, and malonic dialdehyde by the method of EG Korobeynikov. The degree of antioxidant system activity was estimated by the content of enzymes — superoxide dismutase by the method of R. Fried, catalase by the method of R.Holmes, C. Masters, glutathione peroxidase — by the method of OG Arkhipova. Statistical processing of the obtained data was carried out according to the Student’s method.
Conclusions. So, the research of the functional state of the prooxidant system in stomach and lungs of animals in different periods of combined experimental asthma and ulcer showed their gradual increasing with the greatest severity in the 33rd day. It might indicate the intensive accumulation of free radical oxidation products and initial compensatory growth of antioxidant system enzymes. In the later period of EBA and ESU modeling (26th, 33rd days) there is an elevation in the lipoperoxidation on the background of AOS depression, which can be seen as the result of depletion of compensatory reactions aimed at decontamination of LPO products. This leads to a violation of cellular homeostasis and the development of oxidative stress.