| Today, the issue of opioid use and dependence is extremely acute and is being addressed in the field of public health due to its widespread use. In Ukraine, according to official data from the Ministry of Health and the Ministry of Internal Affairs, there are 150,000 drug users, about 70% of whom use opioids, mostly under the age of 35. Goal. The aim of our study was to study the ultrastructural organization of the laryngeal mucosa and cartilage on the 21st and 28th days of opioid exposure. Methods. The material of the study were sexually mature, outbred rats males in the number of 32 animals, weighing 80 g, aged 4.5 months. Before collecting necropsy material, the animal was removed from the experiment with diethyl ether. The mucosa and cartilage of the rats larynx were used as material for ultrastructural examination. Ultrastructural capsule were prepared according to conventional methods. Results. As a result of the collection of experimental material after 21 days in patients exposed to an opioid analgesic at a dose of 20 mg kg at the ultrastructural level revealed an increase in dyscirculatory changes, which were most pronounced in the vessels of the laryngeal mucosa. In particular, the lumens of many hemocapillaries were enlarged, overflowing with erythrocytes, most of which were deformed. As a result of the collection of experimental material after 28 days in shurs under the influence of opioid analgesic at a dose of 25 mg kg at the ultrastructural level revealed an increase in alternative changes of epithelial cells, increased polymorphonuclear infiltration of the lamina propria mucosa and submucosal base processes (mainly stasis and redness) in the vessels of different parts of the larynx. Conclusion. At the end of the 21st day of the experimental opioid effect in rats, at the ultrastructural level revealed the progression of dyscirculatory changes, which were most pronounced in the vessels of the lamina propria of the laryngeal mucosa. Degenerative changes developed in some endotheliocytes, which were accompanied by destruction of their mitochondrial crystals and homogenization of the matrix. In the venous channel dyscirculatory changes were more pronounced than in the arterial. Degenerative changes progressed in the chondrocytes of the cartilaginous tissue of the epiglottis. Alternative changes in hyaline cartilage chondrocytes were also observed. At the end of the 28th day of the experimental opioid effect in rats, at the ultrastructural level, warming of alternative changes of epithelial cells, intensification of polymorphonuclear infiltration of the mucosal plate and submucosal plate, as well as pronounced dyscirculatory processes (mainly stasis and hyperemia) were detected.Key words: larynx, electron microscopy, opioid effect, rat. |
При лікуванні захворювань пародонта для досягнення стабізації патологічного процесу та ліквідації його на ранніх стадіях велике значення має проведення своєчасної комплексної терапії. Враховуючи це, розробка більш ефективних методів медикаментного лікування представляє значний практичний і теоретичний інтерес.