Aim: To study the process of hemoglobin oxidation and the enzymatic reactions associated with it.
Materials and Methods: Heparinized human blood (15 IU/ml) was obtained from the clinical department. The concentration of oxy- and methemoglobin,
auto-oxidation of hemoglobin was determined spectrophotometrically spectrophotometrically. Autooxidation of hemoglobin was recorded spectrophotometrically, and protein concentration was determined by the Lowry method. Monooxygenase activity of hemoglobin was also measured by the method described
by Lowry spectrophotometrically. The concentration of O2 and H2O2 in the reaction media was determined on a biomicroanalyzer OR 210/3 (Redelkis).
Results: The obtained experimental data allow us to propose a mechanism of “spontaneous autooxidation” of oxyhemoglobin, which can be described by
the following equations:
Hb2+O2 = Hb3+ + O2 (1)
Hb2+O2 + 2e- + 2H+ = Hb3+ + H2O2 (2)
Hb2+O2 + 2e - + 2H+ = Hb2+ + H2O2 (3)
Hb2+ + O2 = Hb2+O2 (4)
Spectral characteristics of the process of “spontaneous auto-oxidation” indicate the formation of a metform of hemoglobin, the depletion of oxygen by the system was established, at pH 5.6, an increase in the monooxygenase activity of hemoglobin is observed 3-4 times compared to the physiological level.
Сonclusions: In addition to the main, previously known functions of hemoglobin (gas transport, peroxidase, monooxygenase), it catalyzes a two-electron
oxidase reaction in which O2
is reduced to H2O2. This is confirmed by experimental data on the formation of one of the products of “spontaneous autoxidation”
of oxyhemoglobin _ deoxyform at pH 5.6 _ 8.9.
KEY WORDS: oxyhemoglobin, methemoglobin, autooxidation, monooxygenase activity, oxidase reaction, ligands
Taking into account actuality of psoriasis morbidity problem, specialists point out differentiated individual approach to diagnostic and therapeutic stages as a key aspect in the management of patients. Investigation of main points of etiopathogenesis and elaboration of target influence on its key aspects will enable to achieve a set goal. Both rapid epidermal proliferation and dermal inflammatory infiltration are accompanied by numerous formations of new blood vessels, which start during the early changes of psoriasis and vanish after skin lesion clearance. These observations highlighted that angiogenesis is the chief distinguishing feature during the pathogenesis of psoriasis. Among non-specific stimulants of angiogenesis, matrix metalloproteinase (MMP) should be singled out, which is a group of matrix-destructing enzymes, the source of which are fibroblasts, macrophages, neutrophils and other cells that play an important role in tissue remodelling, including neoangiogenesis processes.
Increasing incidence of dermatosis at the background of reduced immunological response to the impact of exo- and endogenous pathogenic agents dictate the necessity of a more profound research of the problem. In addition, antibiotic and immunosuppressive therapy led to activation of saprobic and conventionally pathogenic microflora which is believed to play a prevalent role in the development of dermatosis and, in particular, psoriasis.
Today, platelet-rich plasma is most frequently used in dermatology as an additional method of treatment for different types of alopecia, post-acne, acne, angioneurosis, acute and chronic ulcers with different etiology (diabetic, venous, traumatic, etc.), secondary hyperpigmentation spots and various cosmetic skin defects.
The blood plasma is able to provide a large amount of growth factors and various proteins that can stimulate the healing process. Plasma therapy accelerates neovascularization, increases blood supply and supply of nutrients necessary for cell regeneration in damaged tissue. Plasma therapy stimulates the proliferation and differentiation of cells involved in the healing process.
According to some authors, the prevalence of onychomycosis in the general population is 10- 20%, it increases with age and is 31% in people aged over 60 years. This affects the quality of life of patients, the risk of infecting contact persons and social adaptation. The treatment of such patients is long-term and not always effective due to concomitant pathology, which should be taken into consideration and corrected by the complex therapy for patients with onychomycosis.