Applied Nanoscience 

Abstract

The biocompatibility of NPs to blood cells is a key issue when these NPs are planned for intravenous application because of potential contact with blood cells and proteins. In this work, γ-Fe₂O₃ NPs (~ 9 nm) and their poly(N,N-dimethylacrylamide) (PDMA) and SiO₂-coated derivatives (γ-Fe₂O₃@PDMA and γ-Fe₂O₃@SiO₂, respectively) were investigated. It was detected that both PDMA and SiO₂ coatings decreased NPs’ aggregation in the buffer solutions, as well as in cell culture medium. Neither neat γ-Fe₂O₃ NPs nor their coated derivatives possessed hemolytic activity toward red blood cells. There was no significant loss of body weight observed after the intravenous injection to laboratory mice. The immune response to the injected NPs was assessed by the ELISA measuring. No antibodies of the IgM class were detected, which suggests lack of acute inflammation. On the 35th day of the experiment, there was a rise in the content of the anti-OVA IgG noticed in all three types of the NPs; however, this rise was lower compared to that induced by the positive control. The injected NPs were found to be spread and settled in the pouch cavity, and none of the tested NPs caused vascular damage or distinct signs of inflammation. Summarizing, γ-Fe₂O₃ NPs coated with the PDMA or SiO₂ manifested good compatibility with blood cells in in vitro and in vivo investigations.

Keywords Maghemite · Nanoparticles · Poly(N,N-dimethylacrylamide) · Silica · Hemolytic action · Immune response

The effects of natural clinoptilolite originated from the Transcarpathian region in the Western Ukraine and its composites doped with metal ions were studied toward: (1) cultured pseudo-normal mammalian cell and murine macrophages; (2) neutrophils of blood of healthy human donors; (3) mice immunized with model antigen; (4) mice under air-pouch model for estimation of microvasculature damage; (5) Candida albicans fungi. Silver doping enhanced cytotoxic action of natural clinoptilolite, while zinc doping did not do that. Clinoptilolite-Ag(NH₃)²⁺ was non-toxic for murine macrophages and moderately toxic for human HEК293 cells. Toxicity of clinoptilolite-Ag⁺ composite toward HEК293 cells was comparable with the effect in positive control. Natural clinoptilolite and its silver derivatives enhanced the humoral immune response in mice and the levels of antibodies were comparable with such levels at response to standard adjuvant, which, however, damaged the microvasculature in mice. Furthermore, natural and Ag-enriched clinoptilolite were capable of activating neutrophils with a release of neutrophil extracellular traps. Finally, we showed that both clinoptilolite-Ag(NH₃)²⁺ and clinoptilolite-Ag+ possessed much higher antifungal activity toward Candida albicans compared to such activity of the nonmodified clinoptilolite, while their doping with zinc did not show such enhancement. Thus, the Transcarpathian clinoptilolite possesses low toxicity toward mammalian cells and activates neutrophils in vitro, while silver doping enhanced the cytotoxicity of this material. Silver-doped derivatives demonstrated stimulating action on antibody production and the antifungal effect. Thus, the developed clinoptilolite-based composites are perspective for use as novel natural immuno-stimulators and antifungal agents.

 Keywords Clinoptilolite · Cytotoxicity · Immune-stimulation · Antifungal activity

Засіб у формі гелевої композиції для лікування запальних та дистрофічно-запальних захворювань тканин пародонта, який містить як активний компонент бензидаміну гідрохлорид, який відрізняється тим, що містить бензидаміну гідрохлорид у формі розтертої до порошкоподібного стану таблетки Т-септ та додатково містить як активний компонент протефлазід у складі гелевої основи, що містить альгінат натрію, ніпагін та воду для ін'єкцій, при наступному співвідношенні компонентів, мас. %: альгінат натрію 5,0 ніпагін 0,15 бензидаміну гідрохлорид, що відповідає вмісту 1 розтертої до порошкоподібного стану таблетки Т-септу 0,03 протефлазід 15,0 вода для ін'єкцій 79,82.