Diagnosing the persistent but not clinically pronounced inflammation in periodontal tissues often is an important clinical problem. Measurement of Nitrite-anion concentration in saliva is informative in diagnosing the periodontal pathology at different stages of its development as well as in differentiation of superficial inflammation of periodontal tissues in the case of gingivitis and profound destruction of gums and bone in case of periodontitis.

Aim of our investigations was the examinations of Nitric oxide level in saliva of patients with periodontal pathology by measuring accumulation of Nitrite anion.

Materials and methods. Were examined 47 patients (20-40 years old), including 28 persons with generalized inflammatory diseases of the gums: chronic simple (14) and hypertrophic gingivitis (14) and 19 patients with chronic generalized periodontitis. The level of Nitrite anion in salive of patiens was meassured. Saliva was obtained in the quantity of 1 ml from each patient on an empty stomach.

The method of analysis was as follows: 0,2 ml of sodium hydrocarbonate was added to 0,2 ml of saliva and stored in ice for 10 min, than 0,4 ml of distilled water and 1,2 ml of 4% ZnS04 were introduced into this solution. The mixture was centrifuged for 20 min and 1,2ml of translucent supernatant was separated from it and mixed with 1,2ml of Gries reactive. Results were obtained within 15 min in a spectrophotometry method (λ = 550 nm).

Results. In the patients with simple chronic gingivitis the level of Nitrite anion in saliva differs significantly from the control samples only in the group with exacerbation of simple chronic gingivitis (51,9±2,4 pmoI/L). In the patients with chronic hypertrophic gingivitis amounts of Nitrite anion in saliva were 11,6 ±1,6 pmol/L, but in the case of exacerbation they were increased 4-5 times as much as 56,1 ±2,7 pmol/L. Levels of Nitrite anion increased considerably in the exacerbation of both forms of gingivitis and amounted to 51,9 ±2,4 pmol/L and 56,1 ±2,7 pmol/L, respectively. These results indicate that level of NO synthesis in saliva doesn’t depend on the type of inflammation in the gums (simple or hypertrophic).

All the patients with chronic generalized periodontitis were tested at the active stage of disease and at the stage of remission. Increased amounts of nitrite anion in saliva of the patients with chronic generalized periodontitis were reported at the active stage of chronic

PhOL Ripetska, et al. 681 (pag 680-689)

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generalized periodontitis (84,9 ± 3,8 pmol/L), which exceeds the healthy control group more than 10 times.

Remission in the patients with generalized periodontitis was attained after complex treatment, which included surgical pocket elimination and was characterized with absence of pockets, inflammation and gums bleeding on probing. Levels of nitrite anion in saliva were lowered to 8,5 ±0,7 pmol/L, which makes an insignificant difference with healthy persons (4,9 ±0,6 pmol/L).

Conclusion. The level of nitric-anion which depicts the level of Nitric oxide in the saliva probably depicts not only the activity of periodontal inflammatory process, but also the aggressiveness of alveolar bone destruction.

All human studies were conducted in compliance with the rules of the Helsinki Declaration of the World Medical Association "Ethical principles of medical research with human participation as an object of study". Informed consent was obtained from all participants.

Keywords: gingivitis, periodontitis, Nitric oxide, levels of Nitrite anion in saliva 

 Silicon-containing (Si-containing) polishing paste has been tested clinically in patients with chronic generalized periodontitis, I-st degree of severity. All patients have been examined for the presence of supra- and sub-gingival calculus and bleeding on probing on a six- month-testing time and within one year after conducting the professional hygienic procedures. It has been revealed that the proposed Si-containing polishing paste proved to be effective in the maintenance of gingival health in the lapse of time from 1 to 12 months after treatment. The majority of patients showed neither dental deposits nor bleeding on probing up in the interval from 1 to 12 months after dental scaling and polishing with Si-containing polishing paste. 

 . One of the problems in standardizing Bioadhesive dental film (BDF) produced by the solvent casting method, is the use of a Casting Mould with clearly set geometrical parameters of 10.0 x 60.0 mm, when using a set volume of polymer gel. Many scientific studies dealing with the preparation of polymeric medical film fail to describe the technological equipment necessary for the manufacture of BDF (bio dental films) using the solvent casting method on a flat surface.

During the scientific development of this process, three types of devices were designed, tested and patented, these being a Casting Mould, and Perforating and Cutting Devices to be applied in the production of dental BDF using the solvent casting method.

The Casting Mould is made of a neutral glass and has the dimensions of 60 x 400 mm which allows the tie production of dental films of a standardized size using the solvent casting method. The Perforating Device results in improved perforated BDF in the process of its production. Meanwhile the Cutting Device allows the uniform distribution and cutting of the polymer film into dozaged strips with accurately specified geometrical dimensions of 10.0 x 60.0 mm. 

 The experience of the wide use of beekeeping products by people for health purposes refers modern apitherapy to the category of effective traditional medicine. Among the important areas of apitherapy, dentistry should be noted as a promising area of medical care. 

 The role of pathogenic microorganisms in the development and maintenance of the inflammatory process in periodontal tissues is not in doubt among researchers. One of the most important tasks of clinical periodontology is the antibacterial effect on periodontal pathogens of periodontal pockets. However, there are certain difficulties in implementing this task, associated with the diversity of the microbial composition of periodontal pathogens, and, accordingly, their sensitivity to different antimicrobial drugs.