New S-(ω-fluoralkyl) 4-amino- and 4-acetamidobenzenesulfonothioates were synthesized by the reaction of sodium salts of the substituted thiosulfonic acids and correspondingω-fluoralkylbromides. The study of their antimicrobial activity against thirteen referenceand clinical bacterial and fungal strains using agar diffusion method was conducted. All compounds showed significant antimicrobial activity, two of them showed higher activity against Candida albicans than known antifungal compound of sulfonothioate class, S-ethyl ester of 4-aminobenzenethiosulfonic acid.